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Physiological and transcriptomic responses of Mikania micrantha stem to shading yield novel insights into its invasiveness
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  • Zhaoyang Jiang,
  • Yaping Zheng,
  • Minling Cai,
  • Changlian Peng,
  • Yutao Wang,
  • Weihua LI
Zhaoyang Jiang
South China Normal University
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Yaping Zheng
South China Normal University
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Minling Cai
South China Normal University
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Changlian Peng
South China Normal University
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Yutao Wang
South China Normal University
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Weihua LI
South China Normal University
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Abstract

The rapid stem elongation of the invasive weed Mikania micrantha in the forest understory is of vital significance for its successful invasion. To understand the physiological and molecular mechanisms for this process, here we comparatively investigated the physiological characteristics and transcriptome patterns of M. micrantha stem under low light (30%) and full light (100%) conditions. The results showed that M. micrantha stem had photosynthetic capacity, which was highly plastic to light intensities, constituting of an indispensable part of the plastic response of M. micrantha to shading. M. micrantha had longer internodes, epidermal cells, and consequently longer stems under low light than full light conditions, which could be attributed to the reduced photoprotective substances (flavonoid and anthocyanin) and increased synthesis of phytohormones (gibberellin, GA and Auxin) as observed under shading treatment. The transcriptome sequencing and qPCR verified the results from physiological investigation, and showed that under low light condition the expression levels of genes involving in photosynthesis (e.g. MmPsaA, MmPsbO1 and MmFd3) were generally down-regulated in comparison to full light condition, so were the genes related to the photoprotective substances synthesis (e.g. MmCHS, and MmF3H1) and the negative regulators of phytohormone (e.g. MmAUX1, MmRR1 and MmGAI). It was concluded that the regulation of phytohormones and photoprotective substances are the important material basis for the rapid elongation of M. micrantha stems with high plasticity, which really matters to the vine to have high invasiveness in the forest understory.

Peer review status:Published

26 Jul 2020Submitted to Molecular Ecology
05 Aug 2020Assigned to Editor
05 Aug 2020Submission Checks Completed
12 May 2021Published in Biological Invasions. 10.1007/s10530-021-02546-z